![]() ![]() The additional EST-SSR markers add value to the maps by increasing marker density and provide markers that can be easily transferred to other bermudagrass populations. Many of the SDAFs displayed disomic segregation and thus T89 may be a segmental allotetraploid or an allotetraploid. The T89 and T574 linkage maps spanned 1055 cM and 311.1 cM and include 125 and 36 single-dose amplified fragments (SDAFs), respectively. With the addition of previously generated marker data, 26 T89 linkage groups and eight T574 linkage groups were formed using a log-of-odds (LOD) value of 4.0. Primers were developed from 53 ESTs containing SSRs producing 75 segregating markers from which 28 could be mapped to the T89 and T574 genetic maps. Genetic linkage maps of bermudagrass (Cynodon spp.) species using 118 triploid individuals derived from a cross of T89 and T574 were enriched with expressed sequence tags-derived simple sequence repeat (EST-SSR) markers. ![]() Furthermore, 33 seashore paspalum SSR markers cross-amplified in bahiagrass (Paspalum notatum Flugge) and these markers can be a useful tool in this species. Accessions that are likely polyploids, all with coarse leaves, grouped together whereas accessions with fine-to mid-fine leaves formed a second group. Two major clusters were identified from the seashore paspalum accessions. ![]() A total of 80 SSR markers, including five markers previously developed, were used to assess genetic relationships among 18 Paspalum accessions. The number of simple sequence repeats (SSR) detected in contigs and in singletons was 3511 and 31,949, respectively, and the number of primer sets designed within each group was 9. High throughput sequencing and subsequent assembling of these libraries resulted in 18,967 contigs and 158,595 singletons. In this study, genomic libraries, enriched for microsatellites, were generated using the salt tolerant accession HI33. To identify genetic regions that are associated with salt tolerance, molecular tools must be developed. Some seashore paspalum accessions and cultivars are far more salt tolerant than others. Seashore paspalum (Paspalum vaginatum Swartz) is a warm-season turfgrass that survives in sand dunes along coastal sites, brackish ponds, and in estuaries. The increasing use of secondary water sources for irrigation, which is highly linked to salinization, creates a demand for salt tolerant turf. ![]()
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